Cytotoxicity assay of Pleurotus ostreatus extracts in different cell lines to immunonutritional applications
DOI:
https://doi.org/10.5281/zenodo.10456696Keywords:
pleurotus ostreatus, aqueous extracts, cytotoxicity, cell lines, immunonutritionAbstract
Introduction: bioderivatives proposed as candidates for food ingredients usually require certain evaluations for immunonutritional applications. Edible-medicinal mushrooms are a source of compounds with these potentials. Among them, Pleurotus ostreatus mushrooms contain bioactive metabolites, with important uses in the food industry and in the therapeutic practice of the medical-pharmaceutical industry. In vitro cytotoxicity assays are valuable methods to evaluate products of natural origin, such as fungal extracts.
Objective: to evaluate the cytotoxicity of two extracts obtained from the Pleurotus ostreatus mushroom in different cell lines.
Method: water-soluble extracts were obtained from the mycelium and fruiting bodies of Pleurotus ostreatus in laboratories of the Center for Industrial Biotechnology Studies of the Universidad de Oriente. The cytotoxicity of the bioproducts was evaluated by the resazurin dye reduction assay on three cell lines at the Laboratory of Microbiology, Parasitology and Hygiene (LMPH) of the University of Antwerp, Belgium. Non-adherent THP-1 cells (human leukemia pre-monocytes), Caco-2 adherent cells (human colon adenocarcinoma epithelium) and RAW 264.7 adherent cells (murine macrophages) were used.
Results: Pleurotus ostreatus extracts were not cytotoxic for any of the human or murine cell lines studied, since they did not cause damage to the viability of the epithelial cells of the gastrointestinal system, nor to the immune system cells used.
Conclusions: this result demonstrates that both fungal bioderivatives can be safely applied in immunonutritional studies.
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Copyright (c) 2024 Yamila Lebeque Pérez, Gabriel Llauradó Maury, Manuel de Jesús Serrat Díaz, Paul Cos, Guillermo Antonio Barreto Argilagos
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