Qualitative laserfluorescence in the diagnosis of the cavity limit

Authors

DOI:

https://doi.org/10.5281/zenodo.10136113

Keywords:

laserfluorescence, diagnosis, cavity limit

Abstract

Introduction: laser-induced fluorescence is capable of discerning between healthy and diseased tissue and has been applied to different areas within Dentistry. However, qualitative laserfluorescence has been little studied to determine the cavity limit. Objective: to evaluate the effectiveness and safety of the use of laser fluorescence, at λ=450 nm and 405 nm, in detecting the cavity limit. Method: 45 teeth were selected ex-vivo, which presented at least one location of dentin caries. 83 areas were determined for the study in which fluorescence was measured using three systems with different wavelengths (405-450 nm). The safety of the test was calculated with the color categories recommended by the manufacturer and a scale resulting from the recoding of the variables. Using the Pilxelmator MAC software, the RGB color variables were converted into quantitative values of the CIE scale. The data were entered into the SPSS 19.0 statistical program. Significance tests were applied (α≤0.05). Results: the color categories recommended by the manufacturer do not match the scales that show the best sensitivity and specificity. Laserfluorescence (405 nm-450 nm) is more specific than sensitive. The L*a*b variables are capable of differentiating between recoverable and non-recoverable dentin and the LIF-405 nm discriminates between the layers that delimit the cavity boundary. Conclusions: laser-induced fluorescence is a useful method for diagnosing the cavity limit, although the manufacturer's recommendations must be modified. The L*a*b system can help eliminate operator subjectivity.

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References

1. Henostroza G. Caries Dental, principios y procedimientos para el diagnóstico. 1ed. Lima: Universidad Peruana Cayetano Heredia; 2007.

2. Harris Norman O. Odontología preventiva primaria México México: Manual moderno; 2001.

3. Costerton J.Overview of microbial biofilms. J Ind Microbiol. 1995; 15:137-140. DOI: https://doi.org/10.1007/bf01569816

4. Scheie A, Petersen F. The biofilm concept: consequences for future prophylaxys of oral diseases. Crit Rev Oral Biol Med. 2004; 15: 4-12. DOI: https://doi.org/10.1177/154411130401500102

5. Figueroa-Gordon M, Acevedo A, Alonso G. Microorganismos presentes en las diferentes etapas de la progresión de la lesión de Caries dental. Acta Odonto Venez. 2009; 47: 1-13. Disponible en: http://ve.scielo.org/scielo.php?pid=S0001-63652009000100026&script=sci_abstract

6. Van Houte J, Jordan H V, Laraway R, Kent R, Soparkar PM, Paola PF. Association of the microbial flora of dental plaque and saliva with human root-surface caries. J Dent Res. 1990; 69(8): 1463–8. DOI: https://doi.org/10.1177/00220345900690080301

7. Becker M,Paster BJ, Leys EL, et al. Molecular Analysis of Bacterial Species Associated with Childhood Caries. J Clin Microbiol. 2002; 40:1001-1009. DOI: https://doi.org/10.1128/jcm.40.3.1001-1009.2002

8. Byun R, Nadkarni M, Chhour K, Martin F, Jacques N, Hunter N. Quantitative Analysis of Diverse Lactobacillus Species Present in Advanced Dental Caries. J Clin Microbiol. 2004; 7:3128-3136. DOI: https://doi.org/10.1128/jcm.42.7.3128-3136.2004

9. Lanata E. Operatoria dental: estética y adhesión. 2a ed. Buenos Aires: Grupo Guía; 2003.

10. Kidd EA, Joyston-Bechal S, Beighton D. The use of a caries detector dye during cavity preparation: a microbiological assessment. Br Dent J. 1993; 174(7): 245–8. DOI: https://doi.org/10.1038/sj.bdj.4808142

11. Banerjee A, Watson TF, Kidd EA. Dentine caries excavation: a review of current clinical techniques. Br Dent J. 2000; 188(9): 476–82. DOI: https://doi.org/10.1038/sj.bdj.4800515

12. Kidd E, Ricketts D, Beighton D. Criteria for caries removal at the enamel-dentine junction: a clinical and microbiological study. Br Dent J. 1996; 180:287-291. DOI: https://doi.org/10.1038/sj.bdj.4809066

13. Veitía L,Acevedo A, Rojas F. Métodos convencionales y no convencionales para la detección de lesión inicial de caries, revisión bibliográfica. Acta Odontológica Venezolana. 2011; 49(2): 1-10. Disponible en: https://www.actaodontologica.com/ediciones/2011/2/art-22/

14. Herrera M, Bonilla Represa V, Segura Egea JJ. Caries enfermedad versus caries lesión: implicaciones diagnósticas y terapéuticas según el International Caries Consensus Collaboration Group. Endodoncia. 2016; 34: 204-219.
Disponible en: https://personal.us.es/segurajj/documentos/CV-Art-Sin%20JCR/2016-Endodoncia%20-Caries-Herrera.pdf

15. Innes N, Frencken JE, Bjørndal L, Maltz M, Manton DJ, Ricketts D, et al. Managing Carious Lesions: Consensus Recommendations on Terminology. Adv Dent Res. 2016; 28: 49-57. DOI: https://doi.org/10.1177/0022034516639276

16. Fusayama T. Two layers of carious dentin; diagnosis and treatment. Oper Dent. 1979; 4: 63-70. Disponible en: https://pubmed.ncbi.nlm.nih.gov/296808/

17. Martínez R, Suárez C, Suárez F, Gonzales F. Técnicas de diagnóstico de la caries dental. Boletín de la sociedad de Pediatría de Asturias. 2006;(46): 23-31. Disponible en: https://pesquisa.bvsalud.org/portal/resource/pt/ibc-044162?lang=es

18. Kidd E. How “clean” must a cavity be before restoration? Caries Res. 2004; 38: 305–313. DOI: https://doi.org/10.1159/000077770

19. Margulis D. Photoshop Lab Color: The Canyon Conundrum and Other Adventures in the Most Powerful Colorspace. 1 ed. Peachpit Pr; 2006

20. Labs H. Hunter Lab Color Scale. Insight on Color; 1996.

21. Abalos C, Herrera M, Jiménez-Planas A, Llamas R. Performance of Laser Fluorescence for Detection of Occlusal Dentinal Caries Lesions in Permanent Molars: An in vivo Study with Total Validation of the Sample. Caries Res. 2009; 43(2):137–41. DOI: https://doi.org/10.1159/000209347

22. Abalos C, Mendoza A, Jimenez-Planas A, Guerrero E, Chaparro A, Garcia-Godoy F. Performance of laser fluorescence for the detection of enamel caries in non-cavitated occlusal surfaces: clinical study with total validation of the sample. Am J Dent. 2012; 25(1):44–8. Disponible en: https://pubmed.ncbi.nlm.nih.gov/22558692/

23. Gil-Bermejo C. Validez y Seguridad de la Dureza Clínica vs Láserfluorescencia a λ=655nm, 450nm y 405nm en el diagnóstico del Límite Cavitario: un estudio exvivo con marcadores de ADN. [TFM) us. [Tesis de Maestría]. Sevilla: Universidad de Sevilla; 2018. Disponible en: https://hdl.handle.net/11441/78530

Published

2023-11-15

How to Cite

1.
Laverde-Albarracín DM. Qualitative laserfluorescence in the diagnosis of the cavity limit. Rev Inf Cient [Internet]. 2023 Nov. 15 [cited 2025 Apr. 3];102:4370. Available from: https://revinfcientifica.sld.cu/index.php/ric/article/view/4370

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Section

Original Articles